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li cor odyssey blocking buffer  (LI-COR)


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    LI-COR li cor odyssey blocking buffer
    Li Cor Odyssey Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 2055 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/li+cor+odyssey+blocking+buffer/Intercept+(TBS)+Blocking+Buffer/pm42135436-303-4-4
    Average 98 stars, based on 2055 article reviews
    li cor odyssey blocking buffer - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Comprehensive Proteomic Profiling Reveals Dysregulation of Angiogenesis and Inflammatory Pathways in the Brains of SIRT3 Knockout Mice
    Article Snippet: An equal amount of protein lysates (30 μg) for each sample was separated on 4–12% Bis-Tris NuPAGETM gels (Invitrogen, Carlsbad, CA, USA) and transferred to PVDF membranes (Millipore Sigma, Burlington, MA, USA) using an XCell SureLock mini-cell system (Invitrogen, Carlsbad, CA, USA). .. After transfer, membranes were blocked in a 1:1 solution of Li-COR Odyssey Blocking buffer (Li-COR Biosciences Inc., Linkoln, NE, USA) and PBS. .. The membranes were subsequently probed with an anti-SIRT3 antibody (1:1000, Rabbit Monoclonal Antibody Cat. #5490; Cell Signaling Technology, Boston, MA, USA), and an anti-beta actin antibody (cat. #MA5-15739; Invitrogen) as loading control, overnight in a cold room.

    Article Title: KMT2C and KMT2D amplify GRHL2-driven enhancer activation
    Article Snippet: Samples were then transferred to PVDF membranes using NuPage Transfer Buffer (Thermo Fisher, CAT#NP0006, with added 10% Methanol, NuPage Antioxidant, 0.01% SDS) at 30V in a cold room for either 2 h or overnight before blocking. .. All membranes were blocked, and stained with primaries and secondaries using Li-Cor Odyssey Blocking Buffer (Li-Cor, CAT927-60001) mixed 1:1 with TBS. .. Primary antibodies for western were Rabbit anti-H3 (Cell Signaling, CAT#4493), Mouse anti-alpha Tubulin (Sigma Aldrich, CAT#T-6074), Mouse anti-TBP (Thermo Fisher, CAT#MA1-21516), Rabbit anti-HA (Abcam, CAT#ab9110), Rabbit anti-GRHL2 1:100 (Sigma, CAT#HPA004820), Rabbit anti-KMT2C (provided by Kai Ge), and Rabbit anti-KMT2D (provided by Kai Ge)

    Article Title: Targeting leukemic stem and progenitor cells expressing different BCR::ABL1 levels: antileukemic activity of asciminib with or without TKIs
    Article Snippet: For the ICW assay, 20 × 10 3 leukemic CD34-positive cells were plated in 96-well plates (previously coated with a 100 μg/mL poly-L-lysine solution) and either left untreated or exposed to ASC (1.2 μM), IM (2 μM), NIL (2 μM) or their combination for 24 h in StemSpan Serum-Free Expansion medium (SFEM, Stem Cell Technologies). .. Cells were fixed adding 37% formaldehyde directly into each well (final concentration 4% per well) for 20 min at room temperature with gentle rotation, permeabilized three times for 5 min with 0.1% Triton X-100 in PBS (100μL/well) and blocked with LI-COR Odyssey blocking buffer (LI-COR Biosciences) for 1 h at room temperature. .. Cells were then incubated with a mixture of antibodies containing monoclonal anti-CRKL (clone A1, sc-365471, Santa Cruz) and polyclonal anti-phospho-CRKLY207 (3181S, Cell Signaling Technologies) at 4 °C overnight.

    Article Title: Methylglyoxal-derived glycated albumin enhances the stemness potential of invasive ductal carcinoma-derived breast cancer stem-like cell line KAIMRC1
    Article Snippet: .. Following five washes for 10 min each in TBS-0.1% Tween at RT, membranes were incubated for 1 h at RT with infrared fluorescent secondary antibodies diluted (1:5,000 dilution) in LI-COR Odyssey ® blocking buffer: IRDye 680RD (red)-conjugated goat anti-rabbit or IRDye 800RD (green)-conjugated goat anti-mouse (LI-COR Biosciences) with continuous mixing. .. After washing, protein bands were visualized using the LI-COR Odyssey CLx Scanner, and protein expression levels were quantified using ImageJ software version 1.53e ( https://imagej.net/ij/index.html ) as previously described ( , ).

    Article Title: Bacterial 2',3'-cGAMP activates a SAVED effector to form membrane-disrupting filaments and restrict phage replication.
    Article Snippet: .. For western blot analysis, samples were separated by SDS-PAGE on GenScript SUREPAGE Bis-Tris 4-20% gradient gels followed by transfer to a PVDF membrane using the Bio-Rad Trans-Blot Turbo transfer system, blocking with Li-Cor Odyssey blocking buffer for 1 hour shaking at room temperature, then probing primary antibody overnight at 1:1,000-1:10,000 concentration in TBST overnight at 4 ◦C on an orbital shaker, followed by 3× washes for 15 minutes each with TBST, followed by secondary detection with secondary antibody at 1:10,000 in TBST + 0.01% SDS for one hour at room temperature, followed by 3× washes with TBST, and resuspension of the membrane in 1× TBS. .. The blot was then imaged using a Li-Cor Odyssey CLx Imager.

    Article Title: Membrane curvature elastic stress triggers recruitment of PML-II onto the inner nuclear membrane
    Article Snippet: .. After transfer, nitrocellulose membranes were incubated in LI-COR Odyssey blocking buffer diluted 5:1 (vol/vol) with TBS-Tween (20 mM Tris-HCl [pH 7.4], 150 mM NaCl and 0.1% Tween 20) containing primary antibodies described in the previous section including those against β-actin (A5441; Sigma Aldrich), OSBP ( Lagace et al. , 1999 ) and CEPT1 (N-14; Santa Cruz). .. This was followed by goat anti-rabbit or goat anti-mouse IRDye 800CW and/or IRDye 680LT-labelled secondary antibodies (LI-COR Biosciences).

    Article Title: Comprehensive Proteomic Profiling Reveals Dysregulation of Angiogenesis and Inflammatory Pathways in the Brains of SIRT3 Knockout Mice
    Article Snippet: An equal amount of protein lysates (30 μg) for each sample was separated on 4–12% Bis-Tris NuPAGETM gels (Invitrogen, Carlsbad, CA, USA) and transferred to PVDF membranes (Millipore Sigma, Burlington, MA, USA) using an XCell SureLock mini-cell system (Invitrogen, Carlsbad, CA, USA). .. After transfer, membranes were blocked in a 1:1 solution of Li-COR Odyssey Blocking buffer (Li-COR Biosciences Inc., Linkoln, NE, USA) and PBS. .. The membranes were subsequently probed with an anti-SIRT3 antibody (1:1000, Rabbit Monoclonal Antibody Cat. #5490; Cell Signaling Technology, Boston, MA, USA), and an anti-beta actin antibody (cat. #MA5-15739; Invitrogen) as loading control, overnight in a cold room.

    Article Title: The apical polar ring is essential during the blood stage of Plasmodium falciparum.
    Article Snippet: Proteins were transferred overnight (30 V, 4 °C) to a nitrocellulose membrane (Bio-Rad) using a wet transfer system. .. Membranes were blocked with LI-COR Odyssey blocking buffer for 1 h at RT, probed with primary and IRDye-conjugated secondary antibodies (Supplementary Table 3). .. Blots were imaged on a LI-COR Odyssey CLx imaging system and quantified using LI-COR Image Studio 4.0 (volume measurement tool).

    Staining:

    Article Title: KMT2C and KMT2D amplify GRHL2-driven enhancer activation
    Article Snippet: Samples were then transferred to PVDF membranes using NuPage Transfer Buffer (Thermo Fisher, CAT#NP0006, with added 10% Methanol, NuPage Antioxidant, 0.01% SDS) at 30V in a cold room for either 2 h or overnight before blocking. .. All membranes were blocked, and stained with primaries and secondaries using Li-Cor Odyssey Blocking Buffer (Li-Cor, CAT927-60001) mixed 1:1 with TBS. .. Primary antibodies for western were Rabbit anti-H3 (Cell Signaling, CAT#4493), Mouse anti-alpha Tubulin (Sigma Aldrich, CAT#T-6074), Mouse anti-TBP (Thermo Fisher, CAT#MA1-21516), Rabbit anti-HA (Abcam, CAT#ab9110), Rabbit anti-GRHL2 1:100 (Sigma, CAT#HPA004820), Rabbit anti-KMT2C (provided by Kai Ge), and Rabbit anti-KMT2D (provided by Kai Ge)

    Concentration Assay:

    Article Title: Targeting leukemic stem and progenitor cells expressing different BCR::ABL1 levels: antileukemic activity of asciminib with or without TKIs
    Article Snippet: For the ICW assay, 20 × 10 3 leukemic CD34-positive cells were plated in 96-well plates (previously coated with a 100 μg/mL poly-L-lysine solution) and either left untreated or exposed to ASC (1.2 μM), IM (2 μM), NIL (2 μM) or their combination for 24 h in StemSpan Serum-Free Expansion medium (SFEM, Stem Cell Technologies). .. Cells were fixed adding 37% formaldehyde directly into each well (final concentration 4% per well) for 20 min at room temperature with gentle rotation, permeabilized three times for 5 min with 0.1% Triton X-100 in PBS (100μL/well) and blocked with LI-COR Odyssey blocking buffer (LI-COR Biosciences) for 1 h at room temperature. .. Cells were then incubated with a mixture of antibodies containing monoclonal anti-CRKL (clone A1, sc-365471, Santa Cruz) and polyclonal anti-phospho-CRKLY207 (3181S, Cell Signaling Technologies) at 4 °C overnight.

    Article Title: Bacterial 2',3'-cGAMP activates a SAVED effector to form membrane-disrupting filaments and restrict phage replication.
    Article Snippet: .. For western blot analysis, samples were separated by SDS-PAGE on GenScript SUREPAGE Bis-Tris 4-20% gradient gels followed by transfer to a PVDF membrane using the Bio-Rad Trans-Blot Turbo transfer system, blocking with Li-Cor Odyssey blocking buffer for 1 hour shaking at room temperature, then probing primary antibody overnight at 1:1,000-1:10,000 concentration in TBST overnight at 4 ◦C on an orbital shaker, followed by 3× washes for 15 minutes each with TBST, followed by secondary detection with secondary antibody at 1:10,000 in TBST + 0.01% SDS for one hour at room temperature, followed by 3× washes with TBST, and resuspension of the membrane in 1× TBS. .. The blot was then imaged using a Li-Cor Odyssey CLx Imager.

    Gentle:

    Article Title: Targeting leukemic stem and progenitor cells expressing different BCR::ABL1 levels: antileukemic activity of asciminib with or without TKIs
    Article Snippet: For the ICW assay, 20 × 10 3 leukemic CD34-positive cells were plated in 96-well plates (previously coated with a 100 μg/mL poly-L-lysine solution) and either left untreated or exposed to ASC (1.2 μM), IM (2 μM), NIL (2 μM) or their combination for 24 h in StemSpan Serum-Free Expansion medium (SFEM, Stem Cell Technologies). .. Cells were fixed adding 37% formaldehyde directly into each well (final concentration 4% per well) for 20 min at room temperature with gentle rotation, permeabilized three times for 5 min with 0.1% Triton X-100 in PBS (100μL/well) and blocked with LI-COR Odyssey blocking buffer (LI-COR Biosciences) for 1 h at room temperature. .. Cells were then incubated with a mixture of antibodies containing monoclonal anti-CRKL (clone A1, sc-365471, Santa Cruz) and polyclonal anti-phospho-CRKLY207 (3181S, Cell Signaling Technologies) at 4 °C overnight.

    Incubation:

    Article Title: Methylglyoxal-derived glycated albumin enhances the stemness potential of invasive ductal carcinoma-derived breast cancer stem-like cell line KAIMRC1
    Article Snippet: .. Following five washes for 10 min each in TBS-0.1% Tween at RT, membranes were incubated for 1 h at RT with infrared fluorescent secondary antibodies diluted (1:5,000 dilution) in LI-COR Odyssey ® blocking buffer: IRDye 680RD (red)-conjugated goat anti-rabbit or IRDye 800RD (green)-conjugated goat anti-mouse (LI-COR Biosciences) with continuous mixing. .. After washing, protein bands were visualized using the LI-COR Odyssey CLx Scanner, and protein expression levels were quantified using ImageJ software version 1.53e ( https://imagej.net/ij/index.html ) as previously described ( , ).

    Article Title: Membrane curvature elastic stress triggers recruitment of PML-II onto the inner nuclear membrane
    Article Snippet: .. After transfer, nitrocellulose membranes were incubated in LI-COR Odyssey blocking buffer diluted 5:1 (vol/vol) with TBS-Tween (20 mM Tris-HCl [pH 7.4], 150 mM NaCl and 0.1% Tween 20) containing primary antibodies described in the previous section including those against β-actin (A5441; Sigma Aldrich), OSBP ( Lagace et al. , 1999 ) and CEPT1 (N-14; Santa Cruz). .. This was followed by goat anti-rabbit or goat anti-mouse IRDye 800CW and/or IRDye 680LT-labelled secondary antibodies (LI-COR Biosciences).

    Western Blot:

    Article Title: Bacterial 2',3'-cGAMP activates a SAVED effector to form membrane-disrupting filaments and restrict phage replication.
    Article Snippet: .. For western blot analysis, samples were separated by SDS-PAGE on GenScript SUREPAGE Bis-Tris 4-20% gradient gels followed by transfer to a PVDF membrane using the Bio-Rad Trans-Blot Turbo transfer system, blocking with Li-Cor Odyssey blocking buffer for 1 hour shaking at room temperature, then probing primary antibody overnight at 1:1,000-1:10,000 concentration in TBST overnight at 4 ◦C on an orbital shaker, followed by 3× washes for 15 minutes each with TBST, followed by secondary detection with secondary antibody at 1:10,000 in TBST + 0.01% SDS for one hour at room temperature, followed by 3× washes with TBST, and resuspension of the membrane in 1× TBS. .. The blot was then imaged using a Li-Cor Odyssey CLx Imager.

    SDS Page:

    Article Title: Bacterial 2',3'-cGAMP activates a SAVED effector to form membrane-disrupting filaments and restrict phage replication.
    Article Snippet: .. For western blot analysis, samples were separated by SDS-PAGE on GenScript SUREPAGE Bis-Tris 4-20% gradient gels followed by transfer to a PVDF membrane using the Bio-Rad Trans-Blot Turbo transfer system, blocking with Li-Cor Odyssey blocking buffer for 1 hour shaking at room temperature, then probing primary antibody overnight at 1:1,000-1:10,000 concentration in TBST overnight at 4 ◦C on an orbital shaker, followed by 3× washes for 15 minutes each with TBST, followed by secondary detection with secondary antibody at 1:10,000 in TBST + 0.01% SDS for one hour at room temperature, followed by 3× washes with TBST, and resuspension of the membrane in 1× TBS. .. The blot was then imaged using a Li-Cor Odyssey CLx Imager.

    Membrane:

    Article Title: Bacterial 2',3'-cGAMP activates a SAVED effector to form membrane-disrupting filaments and restrict phage replication.
    Article Snippet: .. For western blot analysis, samples were separated by SDS-PAGE on GenScript SUREPAGE Bis-Tris 4-20% gradient gels followed by transfer to a PVDF membrane using the Bio-Rad Trans-Blot Turbo transfer system, blocking with Li-Cor Odyssey blocking buffer for 1 hour shaking at room temperature, then probing primary antibody overnight at 1:1,000-1:10,000 concentration in TBST overnight at 4 ◦C on an orbital shaker, followed by 3× washes for 15 minutes each with TBST, followed by secondary detection with secondary antibody at 1:10,000 in TBST + 0.01% SDS for one hour at room temperature, followed by 3× washes with TBST, and resuspension of the membrane in 1× TBS. .. The blot was then imaged using a Li-Cor Odyssey CLx Imager.



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